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目的：建立测定血浆中尼美舒利（Ｎｉｍ）的反相高效液相色谱法。方法：血浆样品用甲醇沉淀蛋白，经酸化后加甲苯提取，氮气挥干，加流动相溶解进样。以ＹＷＧＣ１８柱，甲醇０．０５ｍｏｌ·Ｌ－１磷酸二氢钾（５５∶４５）为流动相，ｐＨ５．０２，甲苯磺丁脲为内标，在波长２３０ｎｍ处检测。结果：在０．２６μｇ·ｍｌ－１～１０．８μｇ·ｍｌ－１浓度范围内，峰面积比与浓度呈良好线性关系，ｒ＝０．９９９５。最低检测限为０．２μｇ·ｍｌ－１，方法重现性好，方法回收率为８６．０％～９２．２％，萃取回收率为７２．２％～８８．４％。结论：用本方法测定８例单剂量ｐｏ１００ｍｇＮｉｍ的血药浓度，结果满意。
Objective: To establish a reversed-phase high performance liquid chromatography (HPLC) method for the determination of nimesulide in plasma. Methods: The plasma samples were precipitated with methanol, acidified and extracted with toluene, evaporated to dryness under nitrogen and dissolved in mobile phase. To YWG C18 column, methanol 0.05mol·L-1 potassium dihydrogen phosphate (55:45) as the mobile phase, pH5.02, tolbutamide as internal standard, at a wavelength of 230nm at the detection. Results: In the concentration range of 0.26μg · ml-1 ~ 10.8μg · ml-1, the peak area ratio showed a good linear relationship with the concentration, r = 0.9995. The detection limit was 0.2μg · ml-1. The method was reproducible. The recoveries were 86.0% -92.2%. The extraction recoveries were 72.2% -88.4%. Conclusion: This method was used to determine the blood concentration of eight single-dose po100mgNim, with satisfactory results.