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目的:研究可卡因对大鼠睾丸谷胱甘肽过氧化物酶(glutathione peroxidase GSH-PX)基因表达的影响。方法:性成熟期健康SD雄性大鼠皮下注射可卡因制造吸毒动物模型。化学比色法检测睾丸氧化应激指标;半定量RT-PCR方法检测睾丸组织的GSH-PX mRNA表达。结果:可卡因处理第7天,实验组GSH-PX活性、MDA含量与对照组比较无显著性差异(P>0.05);而GSH-PX mRNA水平显著高于对照组(P<0.05)。可卡因处理第14天,实验组GSH-PX活性显著低于对照组(P<0.05),MDA含量显著高于对照组(P<0.05),而GSH-PX mRNA水平显著高于对照组(P<0.05)。可卡因处理第21天,实验组GSH-PX活性及 mRNA表达水平显著低于对照组(P<0.05),MDA含量显著高于对照组(P<0.05)。可卡因处理第28天,实验组GSH-PX活性及mRNA表达水平显著低于对照组(P<0.05),MDA含量显著高于对照组(P<0.05)。结论:可卡因对大鼠睾丸的损伤作用可能与其抑制GSH-PXmRNA基因表达有关。
Objective: To study the effect of cocaine on gene expression of glutathione peroxidase (GSH-PX) in rat testes. Methods: Male SD rats of sexual maturity were subcutaneously injected with cocaine to make a drug-induced animal model. The oxidative stress index of testis was detected by chemical colorimetry. The expression of GSH-PX mRNA in testis was detected by semi-quantitative RT-PCR. Results: On the 7th day after cocaine treatment, GSH-PX activity and MDA content in the experimental group were not significantly different from those in the control group (P> 0.05). GSH-PX mRNA level in the experimental group was significantly higher than that in the control group (P <0.05). On the 14th day after cocaine treatment, GSH-PX activity in the experimental group was significantly lower than that in the control group (P <0.05), MDA content was significantly higher than that in the control group (P <0.05), while the GSH-PX mRNA level was significantly higher in the experimental group than in the control group 0.05). On the 21st day after cocaine treatment, the GSH-PX activity and mRNA expression in the experimental group were significantly lower than those in the control group (P <0.05). The content of MDA in the experimental group was significantly higher than that in the control group (P <0.05). On the 28th day after cocaine treatment, GSH-PX activity and mRNA expression in the experimental group were significantly lower than those in the control group (P <0.05), and MDA content was significantly higher than that in the control group (P <0.05). Conclusion: The injury of cocaine on rat testes may be related to the inhibition of gene expression of GSH-PXmRNA.