论文部分内容阅读
目的:建立皮质发育障碍模型,探讨皮质发育障碍模型的敏感性。方法:在SD大鼠孕17d腹腔注入1,3-二氯乙烯-亚硝基脲(BCNU)制作皮质发育障碍模型;Nissl染色观察P60d仔鼠病理变化;选取P60d雄性仔鼠,腹腔注射氯化锂-毛果芸香碱,分别比较两组大鼠癫发生的潜伏期、持续状态时间和死亡率。结果:同龄仔鼠脑组织湿重实验组比对照组显著减轻(P<0.01);Nissl染色显示皮质变薄、皮质层次紊乱、海马区域异位细胞异常聚集;有皮质发育障碍的仔鼠注射氯化锂-毛果芸香碱后,癫发生的潜伏期显著缩短(P<0.01),癫持续状态时间延长(P<0.01),死亡率显著升高(P<0.05)。结论:BCNU致皮质发育障碍模型具有癫易感性。
Objective: To establish a model of cortical dysplasia and explore the sensitivity of cortical dysplasia model. Methods: Cortical dysplasia models were made by intraperitoneal injection of 1,3-dichloroethylene-nitrosourea (BCNU) on the 17th day of SD rats. Nissl staining was used to observe the pathological changes of P60d pups. P60d male pups were injected intraperitoneally with chlorinated Lithium - pilocarpine, were compared between the two groups of rats epileptic latency, duration of state and mortality. Results: Compared with the control group, the wet weight of the same age offspring rats in the experimental group was significantly reduced (P <0.01); Nissl staining showed cortical thinning, cortical disorders, abnormal accumulation of ectopic cells in the hippocampus; pups with cortical dysplasia injected with chlorine After lithium-pilocarpine, the incubation period of epilepsy was significantly shortened (P <0.01), the duration of epilepticus prolonged (P <0.01), and the mortality was significantly increased (P <0.05). CONCLUSION: BCNU induced cortical dysplasia model has epilepsy susceptibility.