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目的:探讨致敏的树突状细胞(DC)激活的肿瘤浸润淋巴细胞(TIL)体外抗小鼠乳腺癌活性。方法:从小鼠四肢长骨骨髓中获取DC,应用粒/巨噬细胞集落刺激因子(GM-CSF)、白介素-4(IL-4)和肿瘤全细胞性抗原致敏DC,然后用DC激活TIL,观察TIL在体外对小鼠C127乳腺癌细胞、小鼠MA782乳腺癌细胞和小鼠B16黑色素瘤细胞的杀伤活性。结果:经C127细胞全细胞性抗原致敏的DC激活的TIL具有很强的对C127细胞的杀伤活性[杀伤活性为(70.21±2.86)%],明显高于其对MA782和B16细胞的杀伤活性[杀伤活性分别为(51.31±3.25)%,(31.41±2.65)%],也明显高于未经DC激活的TIL、C127-DC-小鼠脾淋巴细胞和未经DC激活的小鼠脾淋巴细胞对C127细胞杀伤活性[杀伤活性分别为(48.30±2.97)%,(47.76±3.43)%和(17.23±2.56)%]和对MA782细胞杀伤活性[杀伤活性分别为(38.52±2.87)%,(36.62±2.75)%和(18.07±2.40)%]以及对B16细胞杀伤活性[杀伤活性分别为(25.38±2.63)%,(24.82±2.81)%和(17.34±2.81)%],同时B16细胞全细胞性抗原致敏的DC激活的TIL(TIL来源于C127瘤体)也可诱导相对较低的对B16细胞的特异性细胞杀伤活性。结论:经C127细胞全细胞性抗原致敏的DC能诱导TIL产生高效而特异的体外抗小鼠乳腺癌免疫。
Objective: To investigate the anti-mouse mammary cancer activity of sensitized dendritic cells (DCs) activated tumor infiltrating lymphocytes (TILs) in vitro. Methods DCs were harvested from the long bone marrow of the extremities of mice and sensitized DCs with granulocyte / macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4) and tumor whole cell antigen, The killing activity of TIL on mouse C127 breast cancer cells, mouse MA782 breast cancer cells and mouse B16 melanoma cells was observed in vitro. Results: DCs activated by DCs sensitized with whole cell antigen of C127 cells showed a strong cytotoxic activity against C127 cells [(70.21 ± 2.86)%], which was significantly higher than that of CILs on MA782 and B16 cells [(51.31 ± 3.25)%, (31.41 ± 2.65)%], respectively, but also significantly higher than those of TIL without C-DC activation, splenic lymphocytes of C127-DC- The cytotoxicity of the cells to C127 cells were (48.30 ± 2.97)%, (47.76 ± 3.43)% and (17.23 ± 2.56)%, respectively, and the cytotoxic activity against the MA782 cells (38.52 ± 2.87% (36.62 ± 2.75)% and (18.07 ± 2.40)%, respectively] and the killing activity of B16 cells (25.38 ± 2.63%, (24.82 ± 2.81)% and (17.34 ± 2.81)%] Totally DC-primed DC-activated TILs (TIL derived from C127 tumors) can also induce relatively low specific cytotoxic activity against B16 cells. Conclusion: DCs sensitized by whole cell antigen of C127 cells can induce efficient and specific TIL production in vitro against mouse breast cancer.