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建立了放线菌斜面和液体培养物杀虫活性物质简便快速线虫生测法。线虫(Panagrellusredivivus)对Avermectins反应的典型症状是怠-蜷曲,对韶关霉素的典型反应症状是急翻-挺直。6种线虫中P.redivivus和Aphelenchoidescomposticola对Ivermectin较为耐药。测试液中丙酮、乙醇和甲醇浓度低于3%对线虫活力无明显影响。根据对线虫击倒-死亡过程的观察,提出了用击倒中浓度KC50代替致死中浓度LC50更适合速测之需的结论。
A simple and rapid nematode assay was established for actinomycete slope and liquid culture insecticidal active substance. The typical symptom of the Avermectins response of the nematode (Panagrellus redivivus) is lazy-curl, typical of which is euphoria-upright. Six species of nematode P. Both redivivus and Aphelenchoidescomposticola are more resistant to Ivermectin. The concentration of acetone, ethanol and methanol in the test solution of less than 3% had no significant effect on the viability of nematodes. Based on the observation of nematode knockdown - death process, the conclusion that the medium concentration of KC50 should be knocked out to replace the LC50 of lethal medium is more suitable for the quick test.