论文部分内容阅读
Late blight caused by Phytophthora infestans is the most serious disease of tomato production in China. Studies on thegenetics of resistance and identification of molecular markers are very useful for breeding late blight resistant varieties.The objective of this paper was to study the inheritance of late blight resistance and identify simple sequence repeat (SSR)markers associated with resistance allele in tomato (Lycopersicon esculentum Mill). The results came from an F2 progenyof 241 plants derived from a cross between 5# inbred line that is susceptible to late blight and a resistant accessionCLN2037E. The late blight responses of F2 plants were tested by artificially inoculation of detached-leaflets in plate andnatural infection assayed under greenhouse conditions. Both methods showed that the resistance is dominant andinherited as monogenic trait. Genetic mapping and linkage analysis showed that the late blight resistance gene Ph-ROLwas located on chromosome 9 with a genetic distance of 5.7 cM to the SSR marker TOM236.
Late blight caused by the most serious disease of tomato production in China. Studies on the genetics of resistance and identification of molecular markers are very useful for breeding late blight resistant varieties. The objective of this paper was to study the inheritance of late blight resistance and identify simple sequence repeat (SSR) markers associated with resistance allele in tomato (Lycopersicon esculentum Mill). The results came from F2 progeny of 241 plants derived from a cross between 5 # inbred line that is susceptible to late blight and a resistant accession CLN2037E . The late blight responses of F2 plants were tested by artificially inoculation of detached-leaflets in plate and natural infection assayed under greenhouse conditions. Both methods showed that the resistance is dominant and in as linked as monogenic trait. Genetic mapping and linkage analysis showed that the late blight resistance gene Ph-ROL was located on chromosome 9 with a genetic dista nce of 5.7 cM to the SSR marker TOM236.