论文部分内容阅读
以‘洛阳红’牡丹为试验材料,采用RT-PCR方法从其花芽中克隆得到1个开花调控重要转录因子基因FRUITFULL(FUL1)的同源基因,其c DNA开放阅读框长度为768 bp,编码255个氨基酸。序列比对和结构域分析表明,此蛋白具有MADS超家族、K-box超家族的结构域和ARG80多结构域,且具有MADS-box和K-box的稳定保守基序。NCBI blast分析发现克隆得到的氨基酸序列与葡萄中的FUL类蛋白具有较高同源性,为69%。因此将其命名为Ps FUL1,Gen Bank登录号为KX621277。系统进化树分析表明,Ps FUL1与葡萄编码的Vv FUL1亲缘关系最近,属于MADS基因家族中的AP1/FUL亚家族。q RT-PCR表明,Ps FUL1基因在‘洛阳红’牡丹不同组织中表达差异显著,在花芽和花瓣中的表达量最高,苞片和叶片次之,根中最少。在不同品种牡丹(早花‘迎日红’、中花‘洛阳红’、晚花‘花王’)的6个不同开花时期的花瓣中,Ps FUL1基因表达量因花期早晚与开花时期不同而差异显著,表明Ps FUL1基因对牡丹开花及花期的早晚有重要调控作用。
Using ’Luoyanghong’ Peony as test material, a homology gene FRUITFULL (FUL1) which regulates the transcriptional regulation of important transcription factor gene was cloned from its flower bud by RT-PCR. The open reading frame of cDNA was 768 bp, encoding 255 amino acids. Sequence alignment and domain analysis showed that this protein has MADS superfamily, K-box superfamily domain and ARG80 multidomain, and has a stable conserved motif of MADS-box and K-box. NCBI blast analysis showed that the cloned amino acid sequence has a high homology of 69% with the FUL protein in grapes. So named Ps FUL1, Gen Bank accession number KX621277. Phylogenetic tree analysis showed that Ps FUL1 was closest to Vv FUL1 encoded by grape and belonged to AP1 / FUL subfamily of MADS gene family. q RT-PCR indicated that the Ps FUL1 gene was significantly different in different tissues of ’Luoyanghong’ Peony. It had the highest expression in flower buds and petals, followed by bracts and leaves, and the least in roots. In the petals of 6 different flowering stages of different varieties of peony (early flowering red flower, middle flowering ’Luoyang red’, late flowering ’Kao’), the expression level of Ps FUL1 gene was different due to the difference of flowering time and flowering time Significantly, indicating Ps FUL1 gene flowering and flowering of peony early and late have an important regulatory role.