从鲨鱼软骨中提取、纯化出一种新的血管生成抑制因子———鲨鱼软骨血管生成抑制因子 80 (sharkcarti lage derivedangiogenesisinhibitoryfactor 80 ,SCAIF80 ) ,SDS PAGE银染分析显示一条带 ,分子量为 80kD。利用血管内皮细胞增殖、迁移抑制实验 ,证明SCAIF80能显著抑制血管内皮细胞的增殖与迁移 ,均有剂量依赖性。采用鸡胚绒毛尿囊膜分析 ,证明SCAIF80能显著抑制新生血管生成。动物抑瘤实验结果表明SCAIF80显著抑制小鼠肿瘤生长 ,对Lewis肺癌的抑瘤率达 93 .83%。上述结果提示鲨鱼软骨含有一种新的蛋白质 ,可抑制新生血管生成 ,并因此抑制肿瘤生长 A new angiogenesis inhibitor, shark cartilage derived angiogenesis inhibitory factor 80 (SCAIF80), was extracted and purified from shark cartilage. Silver staining analysis on SDS PAGE revealed a band with a molecular weight of 80 kD. Using vascular endothelial cell proliferation and migration inhibition experiments, it was demonstrated that SCAIF80 can significantly inhibit the proliferation and migration of vascular endothelial cells in a dose-dependent manner. The chick embryo chorioallantoic membrane analysis demonstrated that SCAIF80 can significantly inhibit neovascularization. The results of animal tumor inhibition experiments showed that SCAIF80 significantly inhibited tumor growth in mice, and the tumor inhibition rate of Lewis lung cancer was 93.83%. The above results suggest that shark cartilage contains a new protein that inhibits neovascularization and therefore inhibits tumor growth.