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果蝇是遗传学最好的实验材料之一。为解决果蝇观察问题,我们使用了一种方便的果蝇整体装片方法,经二年多存放和观察证明方法简单、效果可靠,值得介绍给老师和同学一试。其方法是先取相应性状的活果蝇(如红眼灰身的雄果蝇和雌果蝇、白眼雄果蝇和雌果蝇、残翅雄果蝇和雌果蝇……)用乙醚麻醉。麻醉方法是将一乙醚棉球放入培养果蝇的培养瓶中,盖上棉塞,一、二分钟即可麻醉(如无乙醚可用少量二甲苯或酒精“麻醉”),在东北地区的冬季放在室外片刻亦可冻昏,置电冰箱冰盒中冷冻片刻亦可。如用乙醚麻醉,最好是待果蝇将醒能
Drosophila is one of the best experimental materials for genetics. In order to solve the problem of fruit fly observation, we used a convenient method of loading whole fruit flies. After more than two years of storage and observation, the method proved to be simple and reliable, and it is worth introducing to the teachers and students. The method is to first take the corresponding traits of live fruit flies (such as the red-eye gray body of male and female flies, white-eye male and female flies, winged male and female flies ... ...) ether anesthesia. The method of anesthesia is to put a cotton ball of diethyl ether into the culture flask of the cultured fruit fly and cover it with a tampon, which can be anesthetized in one or two minutes (if no ether can be used to “narcoticize” with a small amount of xylene or alcohol), in the winter in the northeast region. It can also be frozen in the open air for a while, and can be frozen for a moment in the refrigerator ice box. If ether anesthesia is used, it is best to wait for fruit flies to wake up