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5-羟甲基糠醛(简称5-HMF)与2,4-二硝基苯肼显色,形成稳定的棕红色苯腙化合物,在可见光493nm 波长处有最大吸收;采用双波长薄层扫描法测定其含量,方法简便、快速、准确。
5-Hydroxymethylfurfural (5-HMF for short) and 2,4-dinitrophenylquinone were developed to form a stable brown-red benzoquinone compound with maximum absorption at a visible wavelength of 493 nm; a dual-wavelength thin-layer scanning method was used. To determine its content, the method is simple, rapid, and accurate.