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采用基因重组肿瘤坏死因子（ＴＮＦ）与白细胞介素２（ＩＬ－２）联合诱导急性白血病患者外周血单个核细胞，结果表明：ＴＮＦ（５００Ｕ／ｍｌ）单独不能诱导出ＬＡＫ细胞活性，也不能进一步提高最适剂量ＩＬ－２（１０００Ｕ／ｍｌ）诱导的ＬＡＫ细胞活性，但能显著增强亚适剂量ＩＬ－２（１０～１００Ｕ／ｍｌ）诱导的ＬＡＫ细胞活性。抗ＴＮＦ单抗和抗ＩＬ－２受体β链（Ｐ＿（７５））单抗能显著抑制ＴＮＦ／ＩＬ－２对ＬＡＫ细胞活性的协同诱导作用。ＴＮＦ与ＬＡＫ细胞联合可显著增强对白血病细胞的杀伤活性。
TNF-α and IL-2 were used to induce peripheral blood mononuclear cells in patients with acute leukemia. The results showed that TNF (500U / ml) alone could not induce LAK cell activity, nor could it further The LAK cell activity induced by IL-2 (1000U / ml) was increased, but LAK cell activity induced by IL-2 (10-100U / ml) was significantly increased. Anti-TNF monoclonal antibody and anti-IL-2 receptor beta chain (P_ (75)) monoclonal antibody significantly inhibited the synergistic induction of TNF / IL-2 on LAK cell activity. TNF in combination with LAK cells can significantly enhance the killing activity of leukemia cells.