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应用聚合酶链反应（ＰＣＲ）结合单链构象多态性分析（ＳＳＣＰ）及免疫组化方法研究２８例阴茎癌Ｋ－ｒａｓ基因第１２位密码子点突变和ｒａｓ基因产物Ｐ２１蛋白的表达情况。结果显示２８例阴茎癌发现３例存在Ｋ－ｒａｓ基因第１２位点突变，１２例标本有Ｐ２１蛋白阳性表达，而癌旁组织Ｐ２１表达阴性，并且Ｐ２１阳性表达率与阴茎癌分化程度和临床分期有关。提示ｒａｓ基因的激活在阴茎癌的发生中有一定的作用。首先发现了阴茎癌中Ｋ－ｒａｓ基因第１２位密码子点突变和Ｐ２１蛋白表达阳性。
28 cases of penile cancer K-ras gene mutation in codon 12 and ras gene product P21 protein expression were detected by polymerase chain reaction (PCR) combined with single-strand conformation polymorphism analysis (SSCP) and immunohistochemistry. The results showed that in 28 cases of penile cancer, there were 3 mutations of K-ras gene in 12 cases, 12 cases had P21 protein positive expression, while the adjacent tissue P21 negative expression, P21 positive expression rate and penile cancer differentiation and clinical stage related. Tip ras gene activation in the occurrence of penile cancer have a role. First found in penis cancer K-ras gene codon 12 point mutation and P21 protein expression was positive.