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目的探讨海洋胶原肽与大豆肽混合粉末对老年小鼠的的抗氧化作用。方法用12月龄的健康雌性小鼠,随机分成5组:阴性对照组、阳性对照组、低、中、高剂量组,每组12只。低、中、高剂量组分别灌胃给予0.833 g/kgBW、1.667 g/kg BW、2.500 g/kg BW海洋胶原肽和大豆肽混合粉末,阴性对照组给予蒸馏水,阳性对照组给予0.2g/kg BW VitE。30 d后,称各组老龄鼠的体重并检测各项抗氧化指标:包括血清蛋白质羰基含量、1%的溶血液中丙二醛(MDA)含量、1%溶血液中谷胱甘肽过氧化物酶(GSH-PX)、1%的肝匀浆中超氧化物歧化酶(SOD)活性、血清和肝匀浆中的GSH含量。结果各剂量组老年小鼠的体重并未出现明显改变(P>0.05);中、高剂量组可明显减少老龄小鼠1%溶血液中MDA含量(P<0.01);高剂量组能明显降低血清中蛋白质羰基含量(P<0.05);低剂量组可明显升高老龄小鼠1%肝匀浆中SOD活性和1%溶血液GSH-PX活性(P<0.05);各剂量组血清和肝匀浆中GSH含量均无明显变化。结论海洋胶原肽与大豆肽混合粉末具有抗氧化功能。
Objective To investigate the anti-oxidative effect of marine collagen peptide and soybean peptide mixed powder on aged mice. Methods 12-month-old healthy female mice were randomly divided into 5 groups: negative control group, positive control group, low, middle and high dose group, 12 in each group. 0.833 g / kg BW, 1.667 g / kg BW and 2.500 g / kg BW of marine collagen peptide and soybean peptide mixed powder were respectively given by gavage in low, medium and high dose groups, and the negative control group was given distilled water. The positive control group was given 0.2 g / kg BW VitE. After 30 days, the body weight of the aged rats in each group was measured and the anti-oxidant indexes were measured: including carbonyl content of serum protein, malondialdehyde (MDA) content of 1% hemolysis solution, glutathione peroxide Enzyme (GSH-PX), superoxide dismutase (SOD) activity in 1% liver homogenate and GSH content in serum and liver homogenate. Results The body weight of the aged mice in each dose group did not change significantly (P> 0.05). The middle and high dose groups could significantly reduce the MDA content in the 1% hemolysis blood of the old mice (P <0.01) (P <0.05). The activity of SOD in 1% liver homogenate and GSH-PX activity in 1% lysate of aged mice were significantly increased in low dose group (P <0.05). The levels of serum and liver in each dose group There was no significant change of GSH content in homogenate. Conclusion Marine collagen peptide and soybean peptide mixed powder has antioxidant function.