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目的:研究激光照射对体外培养的婴儿血管瘤内皮细胞相关生长因子及细胞凋亡的影响。方法:活化培养的婴儿血管瘤内皮细胞分为3组，强脉冲光（IPL）组（23 J/cmn 2，照射1次）、激光组（1 064 nm Nd：YAG激光，90 J/cmn 2，照射1次）和对照组（不照射激光）。照射后第1、3、7天，RT-PCR检测血管内皮生长因子（VEGF）、VEGF受体2（VEGFR-2）及碱性成纤维细胞生长因子（bFGF）mRNA的表达，Western印迹检测VEGFR-2蛋白表达，ELISA检测细胞上清液中VEGF和bFGF的含量，流式细胞仪检测细胞凋亡。n 结果:与对照组相比，1 064 nm Nd：YAG激光照射后第7天，VEGF mRNA（0.363±0.021比1.000±0.023）、VEGFR-2 mRNA（0.483±0.017比1.001±0.031）、bFGF mRNA（0.402±0.040比1.000±0.004）表达均下降，VEGFR-2蛋白表达下降（0.332±0.055比0.768±0.096），VEGF[（69.389±24.179）ng/L比（334.506±13.084）ng/L]和bFGF[（2.386±0.151）ng/L比（9.165±0.232）ng/L]分泌减少，细胞凋亡率（18.413%±2.654%比4.300%±0.036%）上升，差异均有统计学意义（n P<0.05）。与对照组相比，IPL组照射后第7天VEGF mRNA（0.436±0.041比1.000±0.023）、VEGFR-2 mRNA（0.493±0.037比1.001±0.031）、bFGF mRNA（0.490±0.044比1.000±0.004）表达下降，VEFGR-2蛋白表达下降（0.406±0.037比0.768±0.096），VEGF[（128.858±6.063）ng/L比（334.506±13.084）ng/L]和bFGF[（2.723±0.471）ng/L比（9.165±0.232）ng/L]分泌减少，细胞凋亡率上升（16.597%±1.877%比4.300%±0.036%），差异均有统计学意义（n P<0.05）。n 结论:1 064 nm Nd：YAG激光可能通过调节VEGF/VEGFR-2信号通路上的关键因子，以及细胞凋亡发挥对血管瘤内皮细胞的抑制作用，从而达到治疗血管瘤的作用。“,”Objective:To evaluate the effect of laser irradiation on related growth factors in and apoptosis of n in vitro cultured infantile hemangioma endothelial cells.n Methods:Cultured infantile hemangioma endothelial cells were divided into 3 groups: intense pulsed light (IPL) group irradiated with IPL at a dose of 23 J/cmn 2 for 1 session, laser group irradiated with 1 064-nm Nd:YAG laser at a dose of 90 J/cmn 2 for 1 session, control group receiving no irradiation. On days 1, 3 and 7 after irradiation, RT-PCR was performed to measure the mRNA expression of vascular endothelial growth factor (VEGF) , VEGF receptor 2 (VEGFR-2) and basic fibroblast growth factor (bFGF) , and Western blot analysis to determine VEGFR-2 protein expression, enzyme-linked immunosorbent assay to detect the levels of VEGF and bFGF in the culture supernatant, and flow cytometry to detect cell apoptosis.n Results:Compared with the control group, the laser group showed significantly decreased mRNA expression of VEGF (0.363±0.021 n vs. 1.000±0.023, n P< 0.001) , VEGFR-2 (0.483±0.017n vs. 1.001±0.031, n P=0.001) and bFGF (0.402±0.040 n vs. 1.000±0.004, n P< 0.001) , decreased VEGFR-2 protein expression (0.332±0.055n vs. 0.768±0.096, n P< 0.05) , decreased levels of VEGF (69.389±24.179 ng/Ln vs. 334.506±13.084 ng/L, n P< 0.001) and bFGF (2.386±0.151 ng/Ln vs. 9.165±0.232 ng/L, n P< 0.001) in the culture supernatant, but significantly increased apoptosis rate (18.413%±2.654%n vs. 4.300%±0.036%, n P< 0.01) on day 7 after irradiation. Compared with the control group, the IPL group also showed significantly decreased mRNA expression of VEGF (0.436±0.041n vs. 1.000±0.023, n P< 0.05) , VEGFR-2 (0.493±0.037n vs. 1.001±0.031, n P< 0.05) and bFGF (0.490±0.044n vs. 1.000±0.004, n P< 0.05) , decreased VEGFR-2 protein expression (0.406±0.037n vs. 0.768±0.096, n P< 0.05) , decreased levels of VEGF (128.858±6.063 ng/Ln vs. 334.506±13.084 ng/L, n P< 0.001) and bFGF (2.723±0.471 ng/Ln vs. 9.165±0.232 ng/L, n P< 0.001) in the culture supernatant, but significantly increased apoptosis rate (16.597%±1.877%n vs. 4.300%±0.036%, n P< 0.01) on day 7 after irradiation.n Conclusion:The 1 064-nm Nd:YAG laser may exert a therapeutic effect on hemangioma by inhibiting hemangioma endothelial cells via regulating key factors on the VEGF/VEGFR-2 signaling pathway and cell apoptosis.