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本文研究了放线菌素 D(ACTD)和环己亚胺(CHM)对绿豆(Phaseolus radiatus L.)子叶脱分化过程及其核酸和蛋白质代谢的作用。绿豆子叶切块在附加激动素10毫克/升的Miller 培养基上不脱分化;子叶切块在附加 NAA 4毫克/升和激动素10毫克/升的 Miller 培养基上脱分化。如果在脱分化的培养基上加入不同浓度的 ACTD 或 CHM,可观察到随浓度提高对脱分化的抑制作用加强;随处理时间的延迟抑制作用减弱。ACTD 浓度达15微克/毫升、CHM 浓度达0.5微克/毫升时则完全抑制子叶切块脱分化。测定不同处理、不同培养时期子叶切块中 RNA、DNA 和蛋白质的含量表明,绿豆子叶脱分化过程中,每块组织的 RNA 量显著增加,而 DNA 的量则相对稳定,只在后期略有增加,加 ACTD 和 CHM 脱分化受抑制的子叶切块,其 RNA 量和后期 DNA、蛋白质量的增加也相应受到抑制。
In this paper, the effects of actinomycin D (ACTD) and cycloheximide (CHM) on cotyledon dedifferentiation and nucleic acid and protein metabolism in mung bean (Phaseolus radiatus L.) were studied. Mungbean leaf segments were not dedifferentiated on Miller medium supplemented with 10 mg / L kinetin; coton cotyledons were detached on Miller medium supplemented with NAA 4 mg / L and kinetin 10 mg / L. If different concentrations of ACTD or CHM were added to the dedifferentiated medium, it was observed that the inhibition of dedifferentiation was enhanced with increasing concentration; the inhibition was delayed with the delay of the treatment time. ACTD concentrations of 15 μg / ml and CHM concentrations of 0.5 μg / ml completely inhibited cotyledonary decellularization. The contents of RNA, DNA and protein in cotyledon cotyledons of different treatments and different culture periods showed that the amount of RNA per tissue significantly increased while the amount of DNA was relatively stable during the process of dedifferentiation of mung bean cotyledons and only slightly increased at the later stage , Plus the cotyledon cotyledons with suppressed dedifferentiation of ACTD and CHM, the amount of RNA and the amount of DNA and protein in the late stage were also inhibited accordingly.