论文部分内容阅读
目的:探讨“渐进式”颅内加压法诱导猪脑死亡对血清中ET-1、TNF-α、IL-1β及IL-6表达的影响。方法:雄性长白猪12头,随机分为两组,对照组:颅内置入颅内压探头及水囊导管,但不进行颅内加压;脑死亡组:颅内置入颅内压探头及水囊导管,根据颅内压和平均动脉压的关系,调整加压强度建立脑死亡模型,并通过呼吸、循环维持脑死亡状态12 h,并检测血清ET-1、TNF-α、IL-1β及IL-6水平。结果:脑死亡组ET-1水平从脑死亡0 h即逐渐升高,TNF-α、IL-1β、IL-6从脑死亡后6 h开始升高,上述因子水平与对照组比较,差异均有统计学意义(P<0.05)。结论:脑死亡时TNF-α、IL-1β、IL-6等炎症因子升高相对迟缓,而ET-1水平发生变化较早,灵敏度高,这可能与其作为前炎症介质有关。
Objective: To investigate the effects of “progressive” intracranial pressurization on the expression of ET-1, TNF-α, IL-1β and IL-6 in pigs induced by brain death. Methods: Twelve male Landrace pigs were randomly divided into two groups. Control group: intracranial pressure catheter and water balloon catheter were inserted intracranially without intracranial pressure. Brain death group: Intracranial pressure probe and water sac According to the relationship between intracranial pressure and mean arterial pressure, the stress intensity was adjusted to establish a brain death model, and the brain death state was maintained by breathing and circulating for 12 h. The levels of ET-1, TNF-α, IL-1β and IL- Level. Results: The levels of ET-1 in brain death group increased gradually from 0 h after brain death, TNF-α, IL-1β and IL-6 increased from 6 h after brain death. The levels of these factors were statistically different from those in control group Significance (P <0.05). CONCLUSION: The inflammatory factors such as TNF-α, IL-1β and IL-6 in brainstem are relatively slow to rise. The ET-1 level changes earlier and has higher sensitivity, which may be related to its role as a proinflammatory mediator.