基于氯霉素(CAP)能强烈抑制Ru(bpy)32+/TPA体系的电化学发光(ECL)信号,结合分子印迹固相萃取(MISPE)样品前处理技术,建立了一种高灵敏度检测牛奶中CAP残留量的方法。在最优实验条件下,体系的ECL猝灭值ΔI与CAP浓度呈良好线性关系,线性范围为1.0×10-13～1.0×10-11g/mL,检测限为3×10-12g/mL,精密度和准确度好,可用于牛奶中CAP残留量的测定。 Based on the fact that chloramphenicol (CAP) can strongly inhibit the ECL signal of Ru (bpy) 32 + / TPA system and combined with the pretreatment of sample by molecular imprinting solid phase extraction (MISPE), a high sensitive detection milk In the CAP residual method. Under optimal experimental conditions, the ECL quenching value ΔI of the system showed a good linear relationship with the CAP concentration. The linear range was 1.0 × 10-13 ~ 1.0 × 10-11g / mL, the detection limit was 3 × 10-12g / mL, Precision and accuracy, can be used for determination of milk CAP residues.