先制备含或不含氯霉素(CAM)的磷脂酰胆碱脂质薄膜,再采用二次非对称离心(dual asymmetric centrifugation,DAC)法进行水合和均质化,制备粒径200~300 nm、适于皮肤给药的脂质体。考察了不同的均质化过程参数以及在均质化前向处方中加入丙二醇(PG)的影响。采用可溶性的b-1,3-(或1,6-)葡聚糖(SBG)为凝胶基质,制备了2.5%的水凝胶,加入所得脂质体后再用DAC法混合,得到均匀的载脂质体凝胶。结果 Firstly, phosphatidyl choline lipid film with or without chloramphenicol (CAM) was prepared and then hydrated and homogenized by dual asymmetric centrifugation (DAC) , Liposomes suitable for dermal administration. Different homogenization process parameters and the effect of adding propylene glycol (PG) to the pre-homogenized formulation were investigated. A 2.5% hydrogel was prepared using a soluble b-1,3- (or 1,6-) dextran (SBG) as the gel matrix, mixed with the resulting liposomes and then mixed by a DAC method to give a homogeneous Of liposome-loaded gel. result