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目的:研究前房灌注高眼压和视神经横断损伤两种模型病理变化的不同。方法:高眼压模型制备采用前房灌注生理盐水法,形成100cmH2O高眼压,诱导大鼠视网膜缺血60min,建立急性青光眼模型;视神经损伤模型制备采用SD大鼠球后视神经切断法。两只方法都是在模型建立7天后断头处死大鼠并取标本,HE染色,光镜下观察视网膜各层厚度及视网膜节细胞形态和数量的变化。结果:两种模型都导致了视网膜节细胞的明显减少;前房加压导致视网膜内层厚度降低,视神经阻断法导致视网膜全层厚度降低。结论:前房灌注法使眼压升高更符合青光眼的病理变化过程,是比较好的模型。
OBJECTIVE: To study the pathological changes in two models of anterior chamber intraocular hypertension and optic nerve transection. Methods: The intraocular pressure (IOP) model was prepared by anterior chamber perfusion of normal saline to form 100cmH2O intraocular pressure to induce retinal ischemia 60min in rats. The model of acute glaucoma was established. The model of optic nerve injury was made by posterior retrobulbar nerve transection. Both methods were performed after the model was established 7 days after decapitation, rats were killed and specimens were taken HE staining, the thickness of retinal layers and the morphology and number of retinal ganglion cells were observed under light microscope. RESULTS: Both models resulted in a significant reduction of retinal ganglion cells; anterior chamber pressure resulted in a decrease in the thickness of the retina and an optic nerve block resulted in a decrease in the thickness of the retina. Conclusion: Anterior chamber perfusion method makes the intraocular pressure more consistent with the pathological changes of glaucoma, which is a good model.