论文部分内容阅读
目的:筛选桃仁-大黄药对不同配比对蓄血证模型大鼠“逐瘀-泻热”作用的最佳配比。方法:间隔24 h 2次大鼠尾静脉注射脂多糖制备蓄血证大鼠模型,给予不同配比的桃仁-大黄药对及抵挡汤全方干预30 d,检测大鼠体温变化、全血粘度、红细胞聚集指数、凝血四项及肝脏、肾脏、睾丸(卵巢)指数等指标;观察肝脏、肾脏组织病理学变化。结果:与正常对照组比较,模型组的平均体温变化值显著增大(P<0.01);全血极低、低、中、高切全血粘度及红细胞聚集指数显著升高(P<0.01);PT、APTT、TT缩短(P<0.01),FIB含量升高(P<0.01);脏器指数差异无统计学意义;肝脏组织大多可见汇管区炎细胞浸润十分严重,肝细胞部分水肿并出现空泡;肾脏组织大多可见部分肾小球毛细血管扩张,肾小管水肿。与模型组比较,除桃仁-大黄(2∶3和3∶2低剂量)组外,给药组平均体温变化值显著减小(P<0.01);全血极低、低、中、高切全血粘度及红细胞聚集指数显著降低(P<0.01);PT、APTT、TT显著延长(P<0.01);FIB含量显著降低(P<0.01);脏器指数差异无统计学意义。桃仁-大黄(1∶1)高剂量组和抵当汤全方高剂量组肝脏汇管区只有少量细胞浸润,肝脏水肿和空泡不显著;桃仁-大黄(3∶2)和(2∶3)高剂量组肾小球毛细血管几乎无扩张,肾小管水肿改善。桃仁-大黄(1∶1)高剂量组和抵当汤全方组肾小球毛细血管和肾小管病变不明显。结论:桃仁-大黄药对不同配比对蓄血证大鼠均有“逐瘀-泻热”作用,以桃仁-大黄(1∶1)配比作用最佳。
OBJECTIVE: To screen out the optimal ratio of peach kernel-rhubarb to the effects of different prescriptions on blood stasis syndrome and blood stasis syndrome in rats. Methods: Rats were injected with lipopolysaccharide (LPS) twice a day for 24 h to establish a rat model of blood transfusion. Rats were given different doses of peach kernel-rhubarb drug and Danshen Decoction for 30 days. The changes of body temperature, whole blood viscosity, erythrocyte aggregation Index, coagulation and liver, kidney, testis (ovary) index and other indicators; observed liver, kidney histopathological changes. Results: Compared with the normal control group, the changes of mean body temperature in the model group were significantly increased (P <0.01), and the whole blood viscosity, erythrocyte aggregation index (P <0.01) ; PT, APTT, TT shortened (P <0.01), FIB content increased (P <0.01); there was no significant difference in organ index; most of the liver tissue showed swollen inflammatory cell in portal area was very serious, partial edema of hepatocytes appeared Vacuolar; most of the kidney tissue can see part of the glomerular capillary dilatation, tubular edema. Compared with the model group, except the peach kernel-rhubarb (2: 3 and 3: 2 low dose) groups, the change of mean body temperature in the administration group was significantly decreased (P <0.01) Whole blood viscosity and erythrocyte aggregation index were significantly decreased (P <0.01); PT, APTT, TT were significantly prolonged (P <0.01); FIB content was significantly lower (P <0.01); There was only a small amount of cell infiltration and hepatic edema and vacuolization in the high-dose Taohe-Rhubarb (1: 1) and high-dose Danzhutang (HFD) groups. Glomerular capillaries almost no expansion, tubular edema improved. Peach - rhubarb (1: 1) high-dose group and the whole group of resistance to the soup group of glomerular capillary and tubular lesions was not obvious. Conclusion: The peach kernel-rhubarb drug has the effect of “stubborn-diarrhea-heat-deficiency” on the rats with different blood stasis syndromes, and the best effect is the ratio of peach kernel-rhubarb (1: 1).