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目的:建立SPE-HPLC-ELSD法测定复方藏药制剂肝纤消颗粒中黄芪甲苷含量的方法。方法:采用固相萃取系统对肝纤消颗粒进行处理,色谱柱为Alltima C18(4.6 mm×250 mm,5μm),流动相乙腈-水(33∶67),流速1.0 mL.min-1,柱温35℃,ELSD漂移管温度110℃,载气流量2.7 L.min-1,进样量20μL。结果:黄芪甲苷在1.014～20.280μg峰面积积分值自然对数与进样量自然对数有良好的线性关系线,r=0.999 3;平均加样回收率为100.1%,RSD 2.6%。结论:该方法操作简单,准确,重复性好,可用于藏药肝纤消颗粒的质量控制。
Objective: To establish a SPE-HPLC-ELSD method for the determination of Astragaloside IV in compound Tibetan medicine preparation. Methods: The liver fibroin pellets were treated with solid phase extraction system. The column was Alltima C18 (4.6 mm × 250 mm, 5 μm), the mobile phase consisted of acetonitrile-water (33:67), the flow rate was 1.0 mL · min- Temperature 35 ℃, ELSD drift tube temperature 110 ℃, carrier gas flow 2.7 L.min-1, injection volume 20μL. Results: The natural logarithm of peak area integral value of astragaloside at 1.014 ~ 20.280μg had a good linear relationship with the natural logarithm of injection volume, r = 0.999 3; the average recoveries were 100.1% and RSD 2.6%. Conclusion: The method is simple, accurate, reproducible and can be used for the quality control of Tibetan medicine Ganxianghua Granules.