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目前用于测定皮质醇的方法不是完全特异的。荧光测定要受皮质酮、脱氧皮质酮和药物的影响;放射免疫测定法的特异性由于抗皮质醇抗体同其他类固醇(诸如皮质素,11-脱氧皮质醇,17-羟孕酮,皮质酮和脱氧皮质酮)有不同程度的交叉反应而受到限制;在竞争性蛋白结合测定法中亦可观察到相似的交叉反应。正常情况下,这些类固醇在血清中的浓度与皮质醇相比是很小的,使得这些测定方法还令人满意。但在新生儿、孕妇及先天性肾上腺皮质增生患者和服用甲吡酮后的血浆中,常存有一定量的与皮质醇起交叉反应的类固醇,正是在这种情况
The current method for measuring cortisol is not completely specific. Fluorescence assays are affected by corticosterone, deoxycorticosterone and drugs; the specificity of radioimmunoassay results from the combination of anti-cortisol antibodies with other steroids (such as cortisol, 11-deoxycortisol, 17-hydroxyprogesterone, Deoxycorticosterone) with varying degrees of cross-reaction was limited; similar cross-reactions were also observed in competitive protein binding assay. Normally, the concentration of these steroids in serum is small compared to cortisol, making these assays satisfactory. However, in newborns, pregnant women and patients with congenital adrenal hyperplasia and metyrapone after plasma, there is often a certain amount of steroid cross-reaction with cortisol, it is in this case