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以天然高分子白及多糖(BSP)为凝胶基质,制备了载黄藤素纳米柔性脂质体(1-FNL)凝胶剂,并考察BSP凝胶对脂质体性质(包封率、体外释放、平均粒径、?电位、酸度值和过氧化值)和人阴道上皮细胞(HVEC)毒性的影响。结果表明,BSP浓度为0.5%~2%时能增大1-FNL的包封率,最大值为83.7%,继续增加BSP浓度至2.5%,包封率反而下降。BSP浓度在0.5%~2.5%范围内能减慢凝胶剂中1-FNL的体外释药速率,且具有浓度依赖性。相较于1-FNL,1-FNL凝胶在30 d内的平均粒径和?电位变化程度小,提示BSP与1-FNL相容性较好。同时,在4、25和40℃贮存30 d期间,凝胶剂的酸度值和过氧化值均低于1-FNL,提示BSP提高了1-FNL的化学稳定性。MTT法及碘化丙啶(PI)荧光染色法表明,BSP能降低1-FNL对HVEC的毒性作用,并减弱1-FNL对细胞膜完整性的影响。
Fibrin-loaded nano-flexible liposomes (1-FNL) gel were prepared by using natural polymer white and polysaccharide (BSP) as gel matrix. The effects of BSP gel on the properties of liposomes (entrapment efficiency, In vitro release, mean particle size,? Potential, acidity and peroxide value) and human vaginal epithelial cells (HVEC) toxicity. The results showed that the entrapment efficiency of 1-FNL was increased when the concentration of BSP was 0.5% ~ 2%, the maximum was 83.7%. When the concentration of BSP was increased to 2.5%, the entrapment efficiency decreased. BSP concentration in the range of 0.5% ~ 2.5% can slow the in vitro release rate of 1-FNL in the gel, and in a concentration-dependent manner. Compared with 1-FNL, 1-FNL gel within 30 d average particle size and potential changes in a small extent, suggesting that BSP and 1-FNL better compatibility. At the same time, the acid value and the peroxide value of gels were lower than 1-FNL at 4, 25 and 40 ℃ storage for 30 d, suggesting that BSP increased the chemical stability of 1-FNL. MTT assay and propidium iodide (PI) staining showed that BSP could reduce the toxicity of 1-FNL to HVEC and attenuate the effect of 1-FNL on cell membrane integrity.