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目的检测急性淋巴细胞白血病(ALL)患者Daxx基因的表达情况。方法采用半定量反转录蛳聚合酶链式反应(RT蛳PCR)技术检测了83例ALL患者(初治41例,复发42例)及36名正常对照Daxx基因的表达。结果41例初治和42例复发患者DaxxmRNA表达阳性率分别为75.61%及71.43%,均高于正常对照组36.11%(P<0.01)。半定量分析结果示,ALL初治和复发组患者的DaxxmRNA表达水平分别为0.43±0.029和0.38±0.025,也高于正常对照组0.27±0.04(P<0.05)。结论Daxx基因在ALL患者中高表达,是否可能与急性淋巴细胞白血病的发病有某种关系值得探讨。
Objective To detect the expression of Daxx gene in patients with acute lymphoblastic leukemia (ALL). Methods RT-PCR was used to detect the expression of Daxx gene in 83 patients with ALL (initial treatment 42 cases, relapse 42 cases) and 36 normal controls. Results The positive rates of Daxx mRNA expression in 41 newly diagnosed and 42 relapsed patients were 75.61% and 71.43%, respectively, which were higher than those of the normal control group (36.11%, P <0.01). Semi-quantitative analysis showed that the expression levels of Daxx mRNA in the naive and relapsed patients were 0.43 ± 0.029 and 0.38 ± 0.025, respectively, which was also higher than that of the normal control group (0.27 ± 0.04, P <0.05). Conclusion The high expression of Daxx gene in ALL patients may be related to the pathogenesis of acute lymphoblastic leukemia.