论文部分内容阅读
作者检查了部分慢性乙型肝炎携带者、1例与3次非甲非乙型输血后肝炎有关的供血者,以及部分肝细胞癌(HCC)患者(他们血清 HBeAg 也为阴性)的外周血淋巴细胞。淋巴细胞经分离和提纯,采用锥虫蓝染色排除试验评价其完整性与活力。然后通过超速离心混匀和分层,用“Auszyme Ⅱ”、“Ausab EIA”、“Corzyme”及“HBe EIA”(Abbott 实验室)等方法检测乙型肝炎标志物。在血清 HBeAg 阴性的慢性乙型肝炎携带者和 HCC 患者的淋巴细胞中 HBeAg 持续存在。有两例值得引起注意。1例为34岁的
The authors examined some patients with chronic hepatitis B, 1 donor blood donor associated with 3 non-B hepatitis B blood transfusions, and peripheral blood lymph nodes in some patients with hepatocellular carcinoma (HCC) whose serum HBeAg is also negative cell. Lymphocytes were isolated and purified using trypan blue exclusion assay to assess their integrity and viability. Hematoxylin and eosin (HE), HepG2, HepG2, HepG2, HepG2, HepG2, HepG2, HepG2, HepG2, HepG2, HBeAg persists in lymphocytes in serum HBeAg-negative Chronic Hepatitis B carriers and HCC patients. Two cases deserve attention. One case was 34 years old