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用精子头部银染法,观察雷公藤多甙及其单体T4、T13、棉酚和微波透热睾丸等抗雄性生育因子,对精子头部异常发生率的影响,以评价其对精子头部异常发生率和对生殖细胞的可能作用。实验表明,雷公藤多甙,单体T13、微波透热睾丸可使精子头部异常发生率明显增加;而棉酚及雷公藤单体T4则无明显增加。对睾丸滴片及睾丸切片的观察也与上述结果相符,表明雷公藤多甙、雷公藤单体T13、微波透热睾丸可能导致生殖细胞产生遗传效应,不宜发展为男性抗生育因子。而棉酚、雷公藤单体T4(雷藤氯内酯醇)则无明显生殖细胞的遗传效应,值得深入研究。
Silver staining of the sperm head was used to observe the effects of anti-male fertility factors such as tripterygium wilfordii and its monomers T4, T13, gossypol, and microwave diathermy testicles on the incidence of sperm head abnormalities to evaluate their effects on sperm heads. Incidence of abnormalities and possible effects on germ cells. Experiments have shown that more tripterygium wilfordii, monomer T13, microwave diathermy testicles can significantly increase the incidence of sperm head abnormalities; and gossypol and Tripterygium T4 monomer no significant increase. Observations on the testicular discs and testis slices were also consistent with the above results, suggesting that Tripterygium wilfordii, tripterygium wilfordii T13, and microwave diathermy testes may cause genetic effects in germ cells, and should not be developed as a male antifertility factor. The gossypol and Tripterygium wilfordii monomer T4 (Letolactone) had no genetic effects on germ cells and deserves further study.