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作者从维生素B_2(RF、FMN)在微量条件下与EDTA发生光化还原反应的速率和含量成线性关系出发,采用光反应动力学法来测定药品、食品及动物组织中的维生素B_2.该法简单、方便,具有较高的灵敏度、准确性、精密度和专属性.方法:先记录不同浓度条件下反应的电流时间曲线,由t=2min的电流值和对应的浓度回归作图得
The author uses the photoreaction kinetics method to determine vitamin B 2 in pharmaceutical, food and animal tissues from the linear relationship between the rate and content of vitamin B 2 (RF, FMN) and photochemical reduction reaction of EDTA under the condition of trace amount. Simple and convenient, with high sensitivity, accuracy, precision and specificity.Methods: The current time curve of the reaction under different concentrations was recorded, and the current value at t = 2min and the corresponding concentration regression were plotted