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The N-terminal sequence of Luffaculin 1 was determined to be Asp-Val-Ser-Phe-Ser-. The CD spectrum of Luffaculin 1 indicated that Luffaculin 1 contains the 37.1% α-helix, 33.4% β-sheet,and 29.5% random coil. N-glycosidase activity of Luffaculin 1 against