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通过体外扩增弓形虫ＲＨ、ＺＳ１、ＺＳ２、ＧＴ１４个分离株的编码棒状体蛋白（ＲＯＰ１）和主要表膜蛋白（Ｐ３０）抗原的基因片段，比较虫株间差异，为克隆及其ＤＮＡ免疫的研究做准备。特定引物的设计；弓形虫虫株的复苏、接种、收集、纯化；提取弓形虫ＲＨ、ＺＳ１、ＺＳ２及ＧＴ１分离株的基因组ＤＮＡ，并以此为模板进行ＰＣＲ扩增，扩增产物经１％琼脂糖凝胶电泳分析。结果从４个分离株基因组ＤＮＡ中均扩增出编码ＲＯＰ１、Ｐ３０抗原的基因片段，其大小ＲＯＰ１约７５６ｂｐ，Ｐ３０约１０２５ｂｐ，电泳条带未见虫株间的明显差别。研究表明，所扩增４个弓形虫分离株的ＲＯＰ１和Ｐ３０基因片段均与理论预测值相符，并具有高度保守性。
The gene fragments encoding ROP1 and P30 antigen of RH, ZS1, ZS2 and GT isolates of Toxoplasma gondii were amplified in vitro, and the differences between the strains were compared to clone and DNA-immunized Research preparation. Specific primers; recovery, inoculation, collection and purification of Toxoplasma gondii strains; genomic DNA of RH, ZS1, ZS2 and GT1 isolates of Toxoplasma gondii were extracted and used as templates for PCR amplification. The amplified products were purified by 1% Agarose gel electrophoresis analysis. Results The gene fragments encoding ROP1 and P30 were amplified from genomic DNA of 4 isolates. The size of ROP1 gene was about 756 bp and P30 was about 1025 bp. There was no obvious difference between the two strains. Studies have shown that the four amplified fragments of Toxoplasma gondii ROP1 and P30 gene fragments are consistent with the theoretical predictions, and highly conserved.