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为了解山东省规模化猪场由猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)和猪伪狂犬病毒(PRV)引起猪病毒性腹泻的流行情况,自2014年1月至2016年12月,对来自山东省各地规模化猪场的猪腹泻病料(共3 035份)进行PCR检测。结果显示,PEDV、PRV和TGEV阳性率分别为67.49%、9.33%和3.29%;3年间,PEDV阳性率在2014年第四季度最低,为48.15%,2015年第四季度阳性率最高,为88.57%,2016年各季度阳性率相对2015年呈波动下降趋势;TGEV阳性率在2014年第四季度最高,为18.52%,2015年第三季度阳性率为15.38%,2016年第一季度阳性率为6.67%,其他季度未检测出阳性病料;PRV阳性率在2016年第二季度最高,为15.68%,除2014年第一季度未检出阳性病料外,2014年第二季度阳性率最低,为2.56%。通过对69份被动送检的病料进行PEDV、TGEV和PRV混合感染检测发现,这部分病料中PEDV、TGEV、PRV阳性率分别为86.96%、5.80%和37.68%;总单独感染率为69.57%,PEDV、TGEV和PRV单独感染率分别为57.97%、1.45%和10.14%;总混合感染率为30.43%,PEDV/PRV、PEDV/TGEV和TGEV/PRV混合感染率分别为26.09%、2.90%和1.45%;总单独感染率比总混合感染率高。结果表明,山东省存在PEDV、PRV和TGEV 3种病毒流行,存在PEDV/TGEV、TGEV/PRV和PEDV/PRV的混合感染,混合感染中主要为PEDV/PRV混合感染,不存在PEDV/PRV/TGEV的混合感染。目前PEDV是引起山东省猪病毒性腹泻的主要病因,本试验结果可为山东省猪病毒性腹泻的诊断和控制提供参考。
To understand the prevalence of swine viral diarrhea in swine farms in Shandong Province due to swine epidemic diarrhea virus (PEDV), swine transmissible gastroenteritis virus (TGEV) and PRV (porcine pseudorabies virus) since January 2014 As of December 2016, PCR products of porcine diarrhea (3 035 samples) from large-scale pig farms in Shandong Province were tested by PCR. The results showed that the positive rates of PEDV, PRV and TGEV were 67.49%, 9.33% and 3.29% respectively. The positive rate of PEDV in the third quarter was the lowest in the fourth quarter of 2014 at 48.15%, the highest in the fourth quarter of 2015 at 88.57 %, The positive rate of each quarter in 2016 showed a downward trend from 2015; the positive rate of TGEV was the highest in the fourth quarter of 2014 at 18.52%; the positive rate was 15.38% in the third quarter of 2015; the positive rate in the first quarter of 2016 was 6.67%. No positive disease materials were detected in the other quarters. The positive rate of PRV in the second quarter of 2016 was 15.68%. The positive rate of PRV in the second quarter of 2014 was the lowest except for the positive disease material in the first quarter of 2014, Is 2.56%. The positive rate of PEDV, TGEV and PRV in this part of cases was 86.96%, 5.80% and 37.68%, respectively. The total infection rate was 69.57 The individual infection rates of PEDV, TGEV and PRV were 57.97%, 1.45% and 10.14%, respectively. The total mixed infection rate was 30.43%, and the mixed infection rates of PEDV / PRV, PEDV / TGEV and TGEV / PRV were 26.09%, 2.90% And 1.45%, respectively; the total infection rate was higher than the total mixed infection rate. The results showed that there were three kinds of viruses of PEDV, PRV and TGEV prevalence in Shandong province, there were mixed infections of PEDV / TGEV, TGEV / PRV and PEDV / PRV. Mixed infection was mainly PEDV / PRV mixed infection without PEDV / PRV / TGEV The mixed infection. At present, PEDV is the main cause of swine viral diarrhea in Shandong Province. The results of this test may provide a reference for the diagnosis and control of swine viral diarrhea in Shandong Province.