The historical development of three generations of enzyme-based amperometric glucose biosensors are compared.A novel design of the first-generation glucose giosensor based on the use of metal-dispersed carbon paste electrode is described.Such a strategy enables remarkable selectivity and improved sensitivity,without excluding or destroying the endogenous electroactive interferences commonly associated with the first-generation glucose biosensor.It offers a strong,preferential electrocatalytic action towards the enzymatically-liberated hydrogen peroxide due to the proper selection of the transducer metal-dispersed material.On the other hand,a unique,non-polar pasting liquid allows efficient operation of the resulting glucose biosensor under severe depletion of oxygen or under a considerable period of thermal stress by providing an internal oxygen supply and establishing a micro-environment of the enzyme against thermoinactivation,respectively.Metal-dispersed carbon paste enzyme electrodes have thus become a promising new design of the first generation biosensors,especially for blood glucose measurements and other biotechnical applications due to their remarkable selectivity,high stability,low oxygen-dependence,and good dynamic performance. The historical development of three generations of enzyme-based amperometric glucose biosensors are compared. A novel design of the first-generation glucose giosensor based on the use of metal-dispersed carbon paste electrode is described. without excluding or destroying the endogenous electroactive interferences commonly associated with the first-generation glucose biosensor. It offers a strong, preferential electrocatalytic action towards the enzymatically-liberated hydrogen peroxide due to the proper selection of the transducer metal-dispersed material. On the other hand , a unique, non-polar pasting liquid allows efficient operation of the resulting glucose biosensor under severe depletion of oxygen or under a substantial period of thermal stress by providing an internal oxygen supply and establishing a micro-environment of the enzyme against thermoinactivation, respectively. Metal-dispersed carbon paste enzyme electrod es have thus become a promising new design of the first generation biosensors, especially for blood glucose measurements and other biotechnical applications due to their remarkable selectivity, high stability, low oxygen-dependence, and good dynamic performance.