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目的:探讨参芎化瘀胶囊对脑缺血再灌注损伤的治疗作用及机制。方法:48只雄性SD大鼠随机分成假手术组、模型组、参芎化瘀胶囊高、低剂量组。改良的Pulsineli4血管阻断(4-VO)法制作全脑缺血模型。术后1d应用电镜和光镜观察海马区脑组织形态变化,术后3～7d水迷宫法测试动物学习记忆功能。结果:与假手术组比较,模型组中海马神经元内细胞器、轴索及毛细血管等超微结构明显受损伤、动物搜索安全岛潜伏期延长(P<0.05);与模型组比较,参芎化瘀胶囊组中大鼠脑组织结构损伤程度减轻、搜索安全岛潜伏期缩短(P<0.05);上述变化在高剂量参芎化瘀胶囊组更为明显。结论:参芎化瘀胶囊对脑缺血再灌注损伤有很好的治疗作用,其机制与减轻脑损伤后脑组织超微结构损害有关。
Objective: To investigate the therapeutic effect and mechanism of Shenxionghuayu capsule on cerebral ischemia-reperfusion injury. Methods: Forty eight male Sprague-Dawley rats were randomly divided into sham operation group, model group and Shenxionghuayu capsule high and low dose groups. Global cerebral ischemia model was made by modified Pulsineli4 vascular occlusion (4-VO) method. The morphological changes of hippocampal area were observed by electron microscope and light microscope 1 d after operation. The water maze test was used to test the animal learning and memory function 3 ~ 7 days after operation. Results: Compared with the sham-operation group, the ultrastructure of hippocampal neurons in the model group was significantly damaged by organelles, axons and capillaries, and the latent period of searching the safe island in the animals was longer (P <0.05). Compared with the model group, In the capsule group, the damage of brain tissue structure was lessened, and the latent period of search for the safety island was shortened (P <0.05). The above changes were more obvious in the high-dose Shenxionghuayu capsule group. Conclusion: Shenxionghuayu Capsule has a good therapeutic effect on cerebral ischemia-reperfusion injury, and its mechanism is related to reducing the ultrastructural damage of brain tissue after brain injury.