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脾气虚证大白鼠骨骼肌的超微结构变化已作了观察研究,发现线粒体数显著减少,其结构改变表现为肿胀、嵴断裂、空泡化等。本文对线粒体的超微结构作进一步图像分析。实验动物Wistar大白鼠,取正常组、脾气虚证组、四君子汤复健组和自然恢复组的骨骼肌用4％戊二醛固定液作前固定,1％四氧化锇固定液后固定,Epon 812包埋。LKB V型超薄切片机切半薄和超薄切片,半薄切片作光学显微镜观察和修块定位,超薄切片用醋酸铀、柠檬酸铅双重染色,日立H-500透射电镜观察照相,Joyce-Loebl μMAGISCAN图像分析仪对四个组线粒体的面积、周长、长度、宽度(见图1)以及长度与宽度之比进行测定、统计分析,对四个组的上述参数分别作t测验。
The ultrastructural changes of skeletal muscle in spleen-qi deficiency rats have been observed and found that the number of mitochondria is significantly reduced, the structural changes manifested as swelling, crista fracture, vacuolization and so on. This article further analyzes the ultrastructure of mitochondria. Experimental animals Wistar rats, normal group, spleen Qi Deficiency group, Sijun Tang Decoction group and natural recovery group skeletal muscle with 4% glutaraldehyde fixed solution for pre-fixed, 1% osmium tetroxide fixed solution after the fixed, Epon 812 package Buried LKB V ultrathin microtome was cut into half thin and ultrathin sections, semi-thin sections were observed under light microscopy and fixed in position. Ultrathin sections were double stained with uranyl acetate and lead citrate, and photographed by Hitachi H-500 transmission electron microscope. Joyce -Loebl μMAGISCAN image analyzer for the four groups of mitochondria area, circumference, length, width ratio (see Figure 1) and the length and width were measured, statistical analysis, the four groups of the above parameters were t test.