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目的探讨醛固酮合酶基因CYP11B2-344C/T、Y染色体HindⅢ酶切位点多态性与原发性高血压的关系。方法对原发性高血压患者654例,正常人386名提取白细胞DNA,聚合酶链反应结合限制性内切酶(HaeⅢ、HindⅢ)方法检测醛因酮合酶和Y染色体基因多态性。结果HindⅢ多态性各基因型差异有统计学意义(P=0.03),HindⅢ(+)基因型收缩压、舒张压均明显降低(P=0.01,P=0.03)。CYP11B2CC、CT基因型与HindⅢ(-)基因型组合时,发生高血压的危险增加1.998倍(P=0.01)。结论Y染色体HindⅢ酶切位点多态性与原发性高血压相关,CYP11B2-344C/T多态性与Y染色体HindⅢ酶切位点多态性可能具有联合作用。
Objective To investigate the relationship between aldosterone synthase gene CYP11B2-344C / T, Y chromosome Hind Ⅲ restriction site polymorphism and essential hypertension. Methods The white blood cell DNA was extracted from 654 cases of essential hypertension and 386 normal subjects. The polymorphisms of aldosterone synthase and Y chromosome were detected by polymerase chain reaction and restriction endonuclease (Hae Ⅲ, Hind Ⅲ). Results The genotypes of Hind Ⅲ polymorphism were significantly different (P = 0.03). The systolic and diastolic blood pressures of Hind Ⅲ (+) genotype were significantly decreased (P = 0.01, P = 0.03). CYP11B2CC, CT genotype combined with HindIII (-) genotype, the risk of developing hypertension increased 1.998 times (P = 0.01). Conclusion The Hind Ⅲ polymorphism of Y chromosome is associated with essential hypertension. CYP11B2-344C / T polymorphism may be associated with polymorphism of Y chromosome Hind Ⅲ restriction site.