论文部分内容阅读
目的:研究石斛酚对高糖损伤的EA.hy926细胞(来源于人脐静脉的永生化内皮细胞系)的保护作用,并探讨其作用机制。方法:采用100 mmol·L-1高糖培养基建立EA.hy926内皮细胞损伤模型,给予石斛酚孵育24 h,采用CCK-8法检测细胞活力,流式细胞术检测细胞凋亡,检测细胞半胱天冬酶-3(Caspase-3)活性以及培养液中SOD(过氧化物歧化酶)、T-AOC(总抗氧化能力)活力和MDA(丙二醛)的含量。结果:石斛酚能抑制Caspase-3活性,降低细胞凋亡率,增强细胞活力,提高SOD和T-AOC活力,减少MDA含量。结论:石斛酚对高糖损伤的血管内皮细胞具有保护作用,其机理与其增强细胞的抗氧化能力密切相关。
Objective: To study the protective effect of gigantol on EA.hy926 cells (immortalized endothelial cell line derived from human umbilical vein) with high glucose and its mechanism. Methods: The injury model of EA.hy926 endothelial cells was established by using 100 mmol·L-1 high glucose medium. The cells were treated with ginkol for 24 hours. Cell viability was detected by CCK-8 assay. Cell apoptosis was detected by flow cytometry. Caspase-3 activity, SOD (superoxide dismutase), T-AOC (total antioxidant capacity) activity and MDA (malondialdehyde) content in the culture medium. Results: GFP inhibited the activity of Caspase-3, decreased the apoptosis rate, increased the cell viability, increased the activities of SOD and T-AOC, and decreased the content of MDA. CONCLUSION: GFP plays a protective role on vascular endothelial cells damaged by high glucose, and its mechanism is closely related to its ability to enhance the antioxidant capacity of cells.