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目的研究黑米皮花色苷(black rice anthocyanins,BRA)对CCl4亚急性肝损伤小鼠的保护作用。方法NIH小鼠60只,随机分为:对照组、CCl4模型组、黑米花色苷低、中、高剂量组(BRA-L0.40g/kgbw、BRA-M0.80g/kgbw、BRA-H1.60g/kgbw)共5组。饲养前3w内,除对照组外的其他4个组动物均腹腔注射四氯化碳(CCl4)玉米油溶液。每周2次,共3w,诱导化学性亚急性肝损伤动物模型。饲养7w后,测定各组动物血清谷丙转氨酶(ALT)和谷草转氨酶水平(AST),血清和肝脏中脂质过氧化产物丙二醛(MDA)含量、抗氧化酶活性及总抗氧化能力,用ELISA法分析BRA对各组动物血清中DNA主要氧化产物8-羟基脱氧鸟苷(8-hydroxy-2’-deoxyguanosine,8-OHdG)的影响,通过HE染色观察肝组织病理学变化。结果摄入BRA后的各剂量组,CCl4诱导的亚急性肝损伤小鼠的ALT、AST活性较模型组显著降低(P<0.05),血清和肝脏中MDA的生成量显著减少(P<0.05),SOD、GSH-Px活性明显增强(P<0.05),肝脏组织的总抗氧化能力(T-AOC)显著增强(P<0.05);摄入高剂量BRA,8-OHdG的含量显著降低(P<0.05);由CCl4引起的肝脏组织气球样变、脂肪变性,炎症浸润等病理学损伤,喂食BRA后,均可得到明显改善。结论BRA对CCl4诱导的亚急性肝损伤具有保护作用,这一作用与其抗氧化活性有关。
Objective To study the protective effect of black rice anthocyanins (BRA) on subacute hepatic injury induced by CCl4 in mice. Methods Sixty NIH mice were randomly divided into control group, CCl4 model group, low-, medium-, high-dose anthocyanins group (BRA-L0.40g/kgbw, BRA-M0.80g/kgbw, BRA-H1. 60g/kgbw) A total of 5 groups. Within 3 weeks before feeding, four other groups of animals except the control group were injected intraperitoneally with carbon tetrachloride (CCl4) corn oil solution. Twice weekly for a total of 3 weeks to induce a chemical subacute liver injury animal model. After feeding for 7 weeks, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, malondialdehyde (MDA) content in serum and liver, antioxidative enzyme activity and total antioxidant capacity were determined in each group of animals. The effect of BRA on 8-hydroxy-2’-deoxyguanosine (8-OHdG), the major oxidation product of DNA in serum of each group of animals, was analyzed by ELISA. The pathological changes of liver tissue were observed by HE staining. Results Compared with the model group, the ALT and AST activity of the subacute hepatic injury induced by CCl4 in each dose group after BRA administration was significantly lower than that of the model group (P<0.05), and the production of MDA in serum and liver was significantly reduced (P<0.05). The activities of SOD and GSH-Px were significantly increased (P<0.05), the total antioxidant capacity of liver tissue (T-AOC) was significantly increased (P<0.05), and the intake of high-dose BRA significantly decreased the content of 8-OHdG (P). <0.05); The liver tissue ballooning, steatosis, inflammatory infiltration and other pathological injuries caused by CCl4 can be significantly improved after feeding BRA. Conclusion BRA has a protective effect on subacute liver injury induced by CCl4. This effect is related to its antioxidative activity.