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目的探讨长期使用氧自由基清除剂维生素C对大鼠老年性聋的影响。方法42只二级Wistar大鼠,随机分成2组:试验组饮用水中加入维生素C;对照组正常进食与饮水。检测2组大鼠内耳丙二醛含量、听性脑干诱发电位(ABR)客观反应阈、耳蜗内mtDNA4834bp缺失的变化。结果(1)大鼠11月龄时,对照组与实验组的ABR反应阈为(49.3±3.9)dBSPL与(49.7±4.4)dBSPL(P>0.05),耳蜗中丙二醛含量为(10.8±1.7)与(11.6±2.1)pmol/g,P<0.05;耳蜗内mtDNA4834bp缺失的发生率比较,差异无统计学意义(P>0.05);(2)大鼠24月龄时,对照组与实验组的ABR反应阈、耳蜗中丙二醛含量及耳蜗内mtDNA4834bp缺失的发生率比较,差异有统计学意义(P<0.01、P<0.01和P<0.05)。结论长期使用氧自由基清除剂维生素C可以减轻大鼠内耳膜脂质过氧化程度,降低大鼠耳蜗内mtDNA4834bp缺失的发生率,从而减轻其老年性聋的程度;氧自由基学说可能是老年性聋的发病机制。
Objective To investigate the long-term use of oxygen free radical scavenger vitamin C on senile deafness in rats. Methods Forty-two Wistar rats were randomly divided into two groups: Vitamin C was added into the drinking water of the experimental group; the control group was fed with normal drinking water. The content of malondialdehyde (MDA), the objective response threshold of auditory brainstem response (ABR) and the deletion of mtDNA4834bp in the cochlea of the two groups were detected. Results (1) At 11 months of age, ABR thresholds in control group and experimental group were (49.3 ± 3.9) dBSPL and (49.7 ± 4.4) dBSPL, respectively. The content of malondialdehyde in the cochlea was (10.8 ± 1.7 and 11.6 ± 2.1 pmol / g, P <0.05. There was no significant difference in the incidence of mtDNA4834bp deletion in the cochlea between the two groups (P> 0.05). (2) ABR threshold, malonaldehyde content in the cochlea, and the incidence of mtDNA4834bp deletion in the cochlea were significantly different (P <0.01, P <0.01, P <0.05). Conclusion Long-term use of oxygen free radical scavenger vitamin C can reduce lipid peroxidation in rat eardrum and reduce the incidence of mtDNA4834bp deletion in the rat cochlea, thereby reducing the degree of senile deafness; oxygen free radical theory may be senile Deaf pathogenesis.