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背景:本实验应用免疫组化及粘液组化方法观察MTS1/p16基因产物在大肠癌组织的表达情况,并与癌旁移行黏膜及正常黏膜进行了对比,了解患者的预后与生活质量。目的:探讨MTS1/p16基因失活与大肠癌发生的关系及对患者生活质量的意义。设计:标准对照的实验研究。地点和对象:地点为中国医科大学附属第二医院外科,对象为2000-01/2001-02住院大肠癌手术患者。男67例,女43例,年龄40～83岁。病程1个月～1.5年,选取患者大肠癌及癌旁组织新鲜标本。方法:应用免疫组化方法观察MTS1/P16基因产物在110例大肠癌组织的表达情况,并与非典型增生、癌旁移行粘膜及正常粘膜进行了比较。主要观察指标:以细胞呈清晰棕色为阳性判断标准,按阳性细胞所占的比例分为:+,阳性细胞数<25%;,阳性细胞数25%～50%;,阳性细胞数>50%。以细胞无棕色或与背景一致的淡棕色为阴性。生活质量测定采用李凌江等编制的生活质量综合评定问卷。结果:癌组织p16阳性分级表达低于正常黏膜及移行黏膜(P<0.05)。在管状腺癌中,高分化22例,p16阳性率86%;中分化43例,p16阳性率33%;低分化7例,p16阳性率29%,高低分化癌之间差异有显著意义(t=5.1,P<0.01)。结论:p16蛋白的表达缺失与大肠癌的发生有关,并随着分化程度下降p16表达缺失增加。该指标对大肠癌患者病情的判
BACKGROUND: In this study, the expression of MTS1 / p16 gene product in colorectal carcinoma was observed by immunohistochemistry and mucohistochemistry. The expression of MTS1 / p16 gene in colorectal carcinoma was compared with the adjacent mucosa and normal mucosa, and the prognosis and quality of life of patients were analyzed. Objective: To investigate the relationship between MTS1 / p16 gene inactivation and colorectal carcinogenesis and its significance to the quality of life of patients. Design: Experimental study of standard control. Location and Subjects: The site is located in the Second Affiliated Hospital of China Medical University, and the target is 2000-01 / 2001-02 hospitalized patients with colorectal cancer surgery. 67 males and 43 females, aged 40 to 83 years. Course of 1 month ~ 1.5 years, select patients with colorectal cancer and adjacent tissue fresh specimens. Methods: The expression of MTS1 / P16 gene product in 110 cases of colorectal carcinoma was observed by immunohistochemistry and compared with that of atypical hyperplasia, paracancerous mucosa and normal mucosa. MAIN OUTCOME MEASURES: The cell was clearly brown as a positive criterion. The percentage of positive cells was: +, the number of positive cells was <25%; the number of positive cells was 25% ~ 50%; the number of positive cells was> 50% . Negative cells with brown or light brown background. Quality of life determination using the preparation of the quality of life of Li Lingjiang comprehensive assessment questionnaire. Results: The positive expression of p16 in cancer tissue was lower than that in normal mucosa and transitional mucosa (P <0.05). In tubular adenocarcinoma, 22 cases were highly differentiated, the positive rate of p16 was 86%; 43 cases were moderately differentiated, the positive rate of p16 was 33%; 7 cases were poorly differentiated, the positive rate of p16 was 29% = 5.1, P <0.01). Conclusion: The loss of p16 protein is associated with the occurrence of colorectal cancer, and the expression of p16 protein is increased with the decreasing of differentiation degree. The indicator of the condition of patients with colorectal cancer