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目的:研究芹菜素(AP)对小鼠T细胞体外增殖、细胞周期和凋亡的影响。方法:无菌分离小鼠淋巴结和胸腺细胞,MTT法检测不同浓度(25、50、100、150、200μmol/L)的AP对多克隆刺激剂刀豆蛋白A(ConA)诱导的T细胞增殖的影响,PI染色结合流式细胞术(FCM)分析细胞周期的分布,Annexin V-FITC/PI双染色结合FCM检测AP对T细胞凋亡的影响,以及AP与地塞米松(DEX)共同作用下T细胞凋亡的变化,并用MTT法检测该药物浓度对T细胞的毒性作用。结果:25~200μmol/LAP对T细胞无药物毒性作用,并明显抑制ConA诱导的T细胞增殖(P<0·01),使细胞周期停滞在G0/G1期,且呈剂量依赖关系;各浓度AP对T细胞凋亡均具有抑制作用,并明显抑制DEX诱导的T细胞凋亡(P<0·01),且呈剂量依赖关系。结论:在一定浓度范围内,AP明显抑制ConA诱导的小鼠T细胞体外增殖,使细胞周期停滞G0/G1期,并明显抑制T细胞凋亡。
Objective: To study the effect of apigenin (AP) on the proliferation, cell cycle and apoptosis of mouse T cells in vitro. METHODS: Mouse lymph node and thymocytes were aseptically isolated, and MTT assay was used to determine the concentration of ConA-induced T cell proliferation with different concentrations (25, 50, 100, 150, 200 μmol/L) of AP. Effects, PI staining combined with flow cytometry (FCM) analysis of cell cycle distribution, Annexin V-FITC/PI double staining combined with FCM detection of AP on T cell apoptosis, and AP and dexamethasone (DEX) together The change of apoptosis of T cells was detected by MTT assay to determine the toxic effect of the drug concentration on T cells. RESULTS: Twenty-five to 200 μmol/LAP had no toxic effects on T cells, and significantly inhibited Con A-induced T cell proliferation (P<0.01), and arrested the cell cycle in G0/G1 phase in a dose-dependent manner. AP inhibited the apoptosis of T cells and significantly inhibited DEX-induced apoptosis of T cells (P<0.01) in a dose-dependent manner. CONCLUSION: In a certain concentration range, AP significantly inhibited Con A-induced proliferation of mouse T cells in vitro, arrested G0/G1 cell cycle arrest, and significantly inhibited T cell apoptosis.