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目的 研究和克隆新的肝癌相关基因 ,探索肝癌发生的分子基础。方法 采用mRNA差异显示技术 ,分析肝细胞癌和癌旁组织间基因的差异表达情况 ,获得差异表达基因片断。用此基因片断作为探针 ,通过筛选胎盘cDNA文库 ,以获得该基因cDNA全长 ,并用Northern印迹杂交的方法 ,分析所获得的新基因在 43对肝细胞癌、对应癌旁组织中的差异表达和在正常组织中的分布。结果 克隆了一个新的肝癌相关基因HCCA2的全长cDNA ,其在人体正常组织中分布较为广泛 ,在正常肝组织中不表达。该基因在 43对肝癌组织中表达的阳性率是 79% (34/ 43)。它的表达与肝癌包膜的完整性、Ki 6 7蛋白的表达相关 (P <0 .0 1)。结论 肝癌差异表达新基因HCCA2可能与肝癌细胞的浸润和增殖能力有关。
Objective To study and clone new liver cancer related genes and explore the molecular basis of liver cancer. Methods mRNA differential display technology was used to analyze the differential expression of genes between hepatocellular carcinoma and adjacent tissues. Differentially expressed genes were obtained. Using this gene fragment as a probe, the placental cDNA library was screened to obtain the full-length cDNA of this gene, and the differential expression of the novel genes obtained in 43 pairs of hepatocellular carcinoma and corresponding adjacent tissues was analyzed by Northern blot hybridization. And distribution in normal tissues. Results A new full-length cDNA of HCCA2, a hepatocarcinogenesis-related gene, was cloned. It was widely distributed in human normal tissues and was not expressed in normal liver tissues. The positive rate of this gene in 43 pairs of liver cancer tissues was 79% (34/43). Its expression was related to the integrity of liver cancer capsule and the expression of Ki 67 protein (P < 0.01). Conclusion The differential expression of HCCA2 in hepatocellular carcinoma may be related to the infiltration and proliferation of hepatoma cells.