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以3年生‘北陆’越橘为试材,对发育过程中果实花青苷含量及合成酶活性变化进行研究,并进一步利用3种紫外光(UV-A、UV-B、UV-C)分别照射转色期越橘果实,测定了果实中花青苷等酚类物质的含量、生物合成酶活性及其基因表达,从底物、酶活性、基因转录水平阐明了不同紫外光对花青苷生物合成途径的影响。3种紫外光都明显诱导了果实花青苷的积累,特别是UV-C照射后果实花青苷含量提高了2.36倍。UV照射显著诱导果实PAL、UFGT酶活性升高,VcPAL、VcUFGT转录增强,但同时也抑制了DFR酶活性及基因表达,其中PAL、UFGT酶活性与花青苷的积累极显著正相关(r=0.807**,r=0.894**),而DFR酶则相反(r=–0.854**)。结果表明,UV照射能诱导丙苯氨酸途径中响应紫外处理的一些关键基因的转录激活(如VcPAL,VcUFGT)或抑制(如VcDFR),并影响相应酶活性的变化,促使花青苷等酚类物质的积累。
The changes of anthocyanin content and synthase activity in fruits of three-year-old ’Hokuriku’ bilberry were studied. Three kinds of UV-A, UV-B and UV- The contents of phenolic compounds and biosynthesis enzymes, such as anthocyanins and their genes, were measured respectively. The effects of different UV-light on the activity of cyanine Glycoside biosynthetic pathway. The three kinds of UV light obviously induced the accumulation of anthocyanin in fruit, especially the anthocyanin content increased by 2.36 times after UV-C irradiation. UFGT, PAL and UFGT activities increased, VcPAL and VcUFGT transcripts were enhanced under UV irradiation, but DFR activity and gene expression were also inhibited. PAL and UFGT activities were positively correlated with anthocyanin accumulation (r = 0.807 **, r = 0.894 **) whereas the DFR enzyme is the opposite (r = -0.854 **). The results showed that UV irradiation could induce transcriptional activation (such as VcPAL, VcUFGT) or inhibition (such as VcDFR) of some key genes in response to UV treatment in the CAMP pathway and affect the changes of corresponding enzyme activities, Accumulation of substances.