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目的探讨介孔二氧化硅(SBA-15)致人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVEC)毒性效应及氧化损伤作用。方法将HUVEC暴露于终浓度分别为0(空白对照)、25、50、100、200μg/ml SBA-15的RPMI 1640培养液中培养24h,采用细胞计数试剂盒(cell counting kit-8,CCK-8)法测定SBA-15作用下HUVEC细胞的生存率;标记了异硫氰酸荧光素(fluorescein isothiocyanate,FITC)的膜联蛋白(Annexin V)和核酸染料碘化丙啶(propidium iodide,PI)双染法检测SBA-15对HUVEC细胞凋亡的影响;二氯荧光乙酰乙酸盐(DCFH-DA)探针法检测SBA-15作用下,细胞活性氧(ROS)水平的变化。结果与空白对照组比较,各浓度SBA-15均对HUVEC的增殖具有抑制作用,且随着SBA-15染毒浓度的升高,细胞生存率明显下降(P<0.05);与空白对照组比较,50、100和200μg/ml SBA-15染毒组HUVEC细胞凋亡率均明显增高(P<0.05);随着SBA-15染毒浓度的升高,细胞ROS水平升高。结论 SBA-15具有内皮细胞毒性作用,可降低HUVEC的生存率;细胞凋亡是SBA-15致HUVEC死亡的方式之一;氧化损伤可能是SBA-15致HUVEC细胞毒性的重要机制。
Objective To investigate the toxic effects and oxidative damage of human umbilical vein endothelial cells (HUVEC) induced by mesoporous silica (SBA-15). Methods HUVECs were cultured in RPMI 1640 medium with final concentrations of 0 (blank control) and 25, 50, 100 and 200 μg / ml SBA-15 respectively. Cell counting kit-8 (CCK- 8) assay was used to determine the survival rate of HUVEC cells treated with SBA-15. Annexin V labeled with fluorescein isothiocyanate (FITC) and propidium iodide (PI) The effects of SBA-15 on the apoptosis of HUVECs were detected by double staining. The changes of reactive oxygen species (ROS) levels by SBA-15 were detected by dichlorofluorescein diacetate (DCFH-DA) Results Compared with the blank control group, all concentrations of SBA-15 had an inhibitory effect on the proliferation of HUVEC, and the cell survival rate was significantly decreased with the increase of SBA-15 concentration (P <0.05). Compared with the blank control group The apoptotic rates of HUVECs in SBA-15 and SBA-15 treated groups were significantly increased (P <0.05). The ROS levels in SBA-15 treated groups were significantly increased with the increase of SBA-15 concentration. Conclusion SBA-15 has endothelial cytotoxicity and can reduce the survival rate of HUVEC. Apoptosis is one of the ways of SUV-15-induced HUVEC death. Oxidative damage may be an important mechanism of SBA-15-induced HUVEC cytotoxicity.