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目的 探讨抑癌基因 p16及细胞周期素 D1 (CyclinD1 )在子宫内膜癌、交界性子宫内膜组织及正常子宫内膜组织中的表达规律及其与子宫内膜癌的关系 .方法 应用免疫组化 SABC法检测 38例子宫内膜癌 ,19例子宫内膜增生过长及不典型增生 ,10例正常子宫内膜组织中基因 p16 ,CyclinD1 的表达 .结果 p16在子宫内膜癌组织中阳性率为 40 % ,在交界性子宫内膜组织中表达阳性率为 6 8% ,在正常子宫内膜组织中无表达 ,正常、交界性及恶性子宫内膜组织中表达阳性率有明显差异 (P<0 .0 5 ) .在子宫内膜癌组织中 p16的表达与病理分级有关 ,与临床分期无明显关联 .Cyclin D1 在子宫内膜癌中呈明显阳性表达 ,表达率 5 7% ,定位于细胞核 ,在交界性子宫内膜病变中阳性率为 2 1% ,正常子宫内膜组织中无表达 ,正常、交界性及恶性子宫内膜组织表达差异明显 (P<0 .0 5 ) .在子宫内膜癌组织中 Cyclin D1 的表达与临床分期及病理分级均无关 ,但有随着分化程度的下降、临床期别的升高而升高的趋势 .结论 p16 ,Cyclin D1 在子宫内膜癌的发生发展过程中具有协同作用 .
Objective To investigate the expression of tumor suppressor gene p16 and cyclin D1 in endometrial carcinoma, borderline endometrium and normal endometrium and its relationship with endometrial carcinoma.Methods Immunohistochemistry The expression of p16 and CyclinD1 in 38 cases of endometrial carcinoma, 19 cases of endometrial hyperplasia and atypical hyperplasia and 10 cases of normal endometrium were detected by SABC method.Results The positive rate of p16 in endometrial carcinoma Was 40%. The positive expression rate in junctional endometrium was 68%, which was not expressed in normal endometrial tissues. The positive expression rates in normal, borderline and malignant endometrial tissues were significantly different (P < 0.05). The expression of p16 in endometrial carcinoma was related to the pathological grade, but not to the clinical stage.Cyclin D1 was obviously expressed in endometrial carcinoma with the expression rate of 57%, located in the nucleus , The positive rate of borderline endometrial lesions was 21%, no expression in normal endometrial tissues, normal, borderline and malignant endometrial tissue were significantly different (P <0.05) .In utero C in cancer tissue The expression of yclin D1 had no correlation with clinical stage and pathological grade, but with the decreasing of differentiation degree and the rising of clinical stage.Conclusion The expression of p16 and Cyclin D1 in endometrial carcinoma has Synergy.