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目的:研究银杏叶提取物对缺氧状态下新生SD乳鼠心肌细胞的影响及其可能机制。方法:新生1天SD乳鼠心肌细胞原代培养并利用氮气培养箱模拟低氧构建乳鼠心肌缺氧体外模型。分为3组处理:对照组,缺氧组,缺氧+药物拮抗组。缺氧时间为12 h,通过免疫组化等检测方法,观察各组心肌细胞的损伤情况及心肌Bcl-2、Bax蛋白表达情况。结果:缺氧可以造成新生SD乳鼠心肌细胞凋亡的发生(hypoxia:75.21%±1.21%,control:1.38%±0.45%,P<0.05,n=20),并导致其表达凋亡抑制因子Bcl-2蛋白水平的显著降低(0.125 fold VS control group,P<0.05),促细胞凋亡因子Bax蛋白水平显著升高(3.011fold VS control group,P<0.05);而银杏叶提取物作用后可明显逆转新生SD乳鼠心肌细胞凋亡的发生(EGb761:23.17%±0.43%,hypoxia:73.13%±1.22%,P<0.05,n=20),并明显逆转Bcl-2(5.716 fold VS hypoxia group,P<0.05)、Bax(0.273fold VS hypoxia group,P<0.05)等蛋白的表达水平。结论:凋亡相关因子Bcl-2和Bax等参与缺氧致心肌损伤过程,导致心肌细胞凋亡,银杏叶提取物能降低心肌Bax表达,提高Bcl-2表达,从而保护心肌细胞,抑制凋亡。
Objective: To investigate the effects of Ginkgo biloba extract on neonatal SD neonatal rat cardiomyocytes under hypoxia and its possible mechanism. Methods: Cardiomyocytes from primary neonatal SD rats were primarily cultured and hypoxia was induced by hypoxia in a nitrogen incubator. Divided into three groups: control group, hypoxia group, hypoxia + drug antagonist group. The duration of hypoxia was 12 h. The injury of myocardial cells and the expression of Bcl-2 and Bax in myocardium were observed by immunohistochemistry and other methods. RESULTS: Hypoxia could induce cardiomyocyte apoptosis in neonatal SD neonatal rats (hypoxia: 75.21% ± 1.21%, control: 1.38% ± 0.45%, P <0.05, n = 20) Bcl-2 protein level was significantly decreased (0.125 fold VS control group, P <0.05), and the level of Bax protein was significantly increased (3.011 fold VS control group, P <0.05); while Ginkgo biloba extract Could significantly reverse the cardiomyocyte apoptosis in neonatal SD neonatal rats (EGb761: 23.17% ± 0.43%, hypoxia: 73.13% ± 1.22%, P <0.05, n = 20) group, P <0.05), Bax (0.273fold VS hypoxia group, P <0.05). CONCLUSION: Bcl-2 and Bax are involved in the process of hypoxia-induced myocardial injury and apoptosis of cardiomyocytes. Ginkgo biloba extract can decrease the expression of Bax and increase the expression of Bcl-2, thus protecting cardiomyocytes and inhibiting apoptosis .