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[目的]探讨TTF1-NP对大鼠原发性肝癌生长的抑制作用及其机制.[方法]采用二乙基亚硝胺(DEN)间断给药法建立大鼠肝癌模型,行HE染色观察TTF1-NP干预后大鼠肝脏的形态学变化;利用Western Blot技术检测TTF1-NP干预后大鼠内质网应激(ERS)CHOP通路主要调控蛋白的表达情况.[结果]TTF1-NP对大鼠原发性肝癌的生长有抑制作用,随着TTF1-NP质量浓度的升高,GRP78,eIF2α,IRE1表达逐渐增加,而CHOP仅在高剂量组表达增加.[结论]TTF1-NP可通过活化ERS的CHOP通路抑制DEN诱发的大鼠原发性肝癌的生长.
[Objective] To investigate the inhibitory effect of TTF1-NP on the growth of primary hepatocellular carcinoma in rats and its mechanism. [Methods] The model of hepatocellular carcinoma was established by intermittent administration of diethylnitrosamine (DEN) NP intervention; Western blot was used to detect the expression of CHOP pathway regulated by endoplasmic reticulum stress (ERS) in rats after TTF1-NP intervention. [Results] TTF1- The expression of GRP78, eIF2α and IRE1 increased gradually with the increase of TTF1-NP concentration, while the expression of CHOP increased only in high dose group. [Conclusion] TTF1-NP can activate ERS CHOP pathway inhibits the growth of DEN-induced primary hepatocellular carcinoma in rats.