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利用协同液相微萃取方法初步探讨氢溴酸山莨菪碱、硫酸阿托品、氢溴酸东莨菪碱和丁溴酸东莨菪碱的优势构象;阐明有机溶剂对4种莨菪碱的萃取选择性和协同萃取作用机理;探讨中空纤维与莨菪碱形成电荷转移超分子对萃取效果的影响以及4种莨菪碱结构中N原子的亲电能力;结合高效液相色谱技术测定药物制剂中4种莨菪碱的含量。4种莨菪碱的线性范围均为0.05～5mg/L(r>0.99),方法RSD小于7%,消旋山莨菪碱片和颠茄片中平均回收率分别为95.0%～119.0%和93.0%～95.0%,氢溴酸山莨菪碱和丁溴酸东莨菪碱检出限为0.03mg/L,硫酸阿托品和氢溴酸东莨菪碱检测出限为0.01mg/L。
The synergic liquid phase microextraction method was used to investigate the dominant conformation of anisodamine hydrobromide, atropine sulfate, scopolamine hydrobromide and scopolamine hydrobromide. The mechanism of extraction selectivity and synergistic extraction of four kinds of hyoscyamine by organic solvents was elucidated. To investigate the effect of charge-transfer supermolecule formed by hollow fiber and hyoscyamine on the extraction and the electrophilicity of N atom in four kinds of hyoscyamine. The content of four kinds of hyoscyamine in pharmaceutical preparations was determined by high performance liquid chromatography (HPLC). The linear range of the four kinds of scopolamine were 0.05 ~ 5mg / L (r> 0.99), the RSD of the method was less than 7%, the average recoveries of racemic anisodamine and belladonna were 95.0% ~ 119.0% and 93.0% ~ 95.0%, the detection limit of anisodamine hydrobromide and scopolamine butudrate is 0.03 mg / L, and the detection limit of atropine sulfate and scopolamine hydrobromide is 0.01 mg / L.