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为探明引进的阿月浑子品种间的亲缘关系,采用RAPD标记技术对引入的12个国外品种和‘新疆优株’进行了分析。用筛选的21条10bp随机引物进行PCR扩增可扩增出137个位点,其中多态位点122个,多态位点比率89.05%;品种间遗传距离在0.2015～0.5163之间,表明各品种间存在一定的遗传差异。UPGMA聚类分析表明,13个阿月浑子品种在遗传距离0.40处可划分为3个类群,第1类包括‘Kerman’、‘Larnaka’、‘M-38’、‘M-P3’、‘Ashoury’、‘N’、‘Joley’、‘Aegina’、‘Avidon’、‘B’和‘Peter’11个品种;第2类为‘Xinjiang selecttree’品种;第3类为‘Mateur’品种。
In order to find out the genetic relationship of the introduced pistachio varieties, the 12 foreign cultivars introduced and the ’Xinjiang superior’ were analyzed by RAPD markers. A total of 137 loci were amplified by PCR using 21 random primers, of which 122 were polymorphic and the percentage of polymorphic loci was 89.05%. The genetic distance between cultivars ranged from 0.2015 to 0.5163, indicating that each There are some genetic differences between varieties. UPGMA cluster analysis showed that 13 pistachio varieties could be divided into three groups at the genetic distance of 0.40. The first group included ’Kerman’, ’Larnaka’, ’M-38’, ’M-P3’, ’ Ashoury ’,’ N ’,’ Joley ’,’ Aegina ’,’ Avidon ’,’ B ’and’ Peter’11 varieties; the second category is ’Xinjiang selecttree’; the third category is ’Mateur’.