将秦艽(Gentiana macrophyllae Pall.)及小秦艽(Gentiana dahurica Fisch.)按照测定龙胆中龙胆苦甙的方法,在Rf约0.4处得红紫色斑点。此斑点和龙胆苦甙的标准品在薄层层板上的结果相一致。为了进一步证实其成分,将干燥的秦艽和小秦艽用甲醇提取。其浓缩液在搅拌下加入丙酮中,得丙酮可溶部分,其蒸发残渣用常法乙酰化,然后通过硅胶柱层析,先用氯仿, Gentiana macrophyllae Pall. And Gentiana dahurica Fisch. Were assayed for gentiopicroside in Gentiana macrocyllae at a Rf of about 0.4 at Rf. This spot is consistent with the results of the gentiopicroside standard on the TLC plate. In order to further confirm its composition, the dried Gentiana macrophylla and Gentiana macrophylla extract with methanol. The concentrate was added to the acetone under stirring to obtain acetone soluble part, the evaporation residue was acetylated with the usual method, and then by silica gel column chromatography, first with chloroform,