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目的通过训练大鼠急进高原模型从肝脏组织形态改变和血中酶谱水平研究训练对大鼠肝脏组织的损伤及防护。方法在海拔1520m环境设实验组(EG)使用中药抗疲Ⅰ号加入常规饲料中喂养,对照组(CG)常规饲料喂养。EG、CG各40只游泳训练4周后,各选30只3h急进至海拔3850m环境静息1.5h,从中各选10只作为静息对照采集标本,EG、CG各20只游泳至力竭后,1、24h采集标本。结果EG与CG相比,血中酶谱含量差异均有显著性。EG肝组织结构正常,肝细胞索结构清晰,核清晰,无变性;CG肝组织结构紊乱,肝细胞索模糊。结论过度训练、改变运动集训环境或进入高海拔地区作业训练更要特别注重加强劳动和训练防护。本防护模型研究通过食品干预训练大鼠,是一种简便易行的方法。
Objective To study the injury and protection of rat liver tissue by training the rats to enter the plateau model from the liver tissue morphological changes and blood zymogram levels. METHODS: The experimental group (EG) was fed at an altitude of 1520 m and fed with traditional Chinese medicine anti-Fatigue I to conventional feed, and the control group (CG) was fed conventional feed. After 40 weeks of training for each of 40 EG and CG swimmers, 30 of them were rushed for 3 hours and 31.5 meters above sea level was rested for 1.5 hours. Ten of them were selected as resting controls. 20 of each EG and CG were swimming until they were exhausted. ,1,24h collection of specimens. Results Compared with CG, there was significant difference in the zymogram contents between EG and CG. The liver tissue structure of EG is normal, the hepatocyte cell structure is clear, the nucleus is clear, and there is no degeneration; the structure of CG liver tissue is disordered and the hepatic cell lines are obscure. Conclusions Over-training, changing the environment of sports training, or entering high-altitude area operations and training must pay more attention to strengthening labor and training protection. This protective model study is a simple and easy method to train rats through food intervention.