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目的研究X染色体失活偏移(SXCI)与女性肺癌发生的关系。方法采集148例女性肺癌患者与289例女性对照者的外周血标本,提取基因组DNA,经HpaⅡ消化后,PCR扩增雄激素受体(AR)基因,产物经变性聚丙烯酰胺凝胶电泳,银染显示结果,比较HpaⅡ消化前后AR基因扩增产物的强度差异,计算其校正比值(CR)。结果采用CR≥10作为SXCI标准时,50岁以下的肺癌患者SXCI发生率高于相同年龄段的对照者(分别为7．9％和1．2％,P=0．046);50岁以上肺癌患者和相同年龄段的对照者SXCI发生率无显著差别(分别为4．5％和5．4％,P=0．488)。无论采用CR≥3抑或≥10为标准,女性肺癌患者中发生SXCI者的平均年龄均比未发生SXCI者提前10岁以上(P<0．05)。结论女性血细胞SXCI与肺癌的早期发生有关。
Objective To study the relationship between X chromosome inactivation shift (SXCI) and female lung cancer. Methods The peripheral blood samples of 148 female lung cancer patients and 289 female controls were collected and genomic DNA was extracted. After digestion with Hpa II, the androgen receptor (AR) gene was amplified by PCR and the product was denatured by polyacrylamide gel electrophoresis. The staining results were shown, and the differences in the intensity of AR gene amplification products before and after HpaII digestion were compared, and the calibration ratio (CR) was calculated. Results When CR≥10 was used as the SXCI criteria, the incidence of SXCI in patients under 50 years of age was higher than in controls of the same age group (7.9% and 1.2%, respectively, P=0.046); lung cancer above 50 years of age. There was no significant difference in the incidence of SXCI between patients and controls of the same age group (4.5% and 5.4%, respectively, P=0.488). Regardless of whether CR≥3 or ≥10 was adopted as the standard, the average age of SXCI in female lung cancer patients was more than 10 years earlier than those without SXCI (P<0.05). Conclusion The female blood cell SXCI is related to the early stage of lung cancer.